Application
Western blot
Sample
Chicken Cell lysate - nuclear (QT6 cells)
Loading amount
7.5 µg
Specification
QT6 cells
Gel Running Conditions
Reduced Denaturing (12% acrylamide)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: 0.5% nonfat dry milk
Secondary antibody
Name
Non-Abcam antibody was used: anti-Mouse IgG
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 16 kDa
Positive control
Whole cell lysate
Additional data
Additional Notes
This experiment was a fractionation to separate cytoplasmic, nuclear, and chromatin fractions.
The concentration of the antibody used was 1:3500
The concentration of the antibody used was 1:3500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 14 2012