Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Rat Cell lysate - other (Chromatin extracted from thymocytes)
Specification
Chromatin extracted from thymocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.1% Tween 20 + 5% BSA
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 15 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
Lane 1: Chromatin extracted from thymocytes, Lane 2: Soluble fraction of chromatin extracted from thymocytes Lane 3: Insoluble fraction of chromatin extracted from thymocytes
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 20 2013