Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Murine embryonic stem cells)
Specification
Murine embryonic stem cells
Treatment
vehicle control (EtOH) or all-trans retinoic acid (RA)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/10000
Secondary antibody
Name
Non-Abcam antibody was used: antiRabbit-IgG, Santa Cruz (sc-2030)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 14 kDa Non-specific: nd kDa
Exposure
10 minute(s) and 0 second(s)
Additional data
PubMed ID
20857416: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Dan Stummer
Verified customer
投稿 Mar 28 2014