Application
Western blot
Sample
Chicken Tissue lysate - whole (thymus homogenates)
Gel Running Conditions
Reduced Denaturing (10-20% Tris/Glycine gel)
Loading amount
10 µg
Specification
thymus homogenates
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% NFDM in TBS-T
Dilution
1/10000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-rabbit IgG Peroxidase Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 14 kDa
Additional data
Additional Notes
ABTRIAL-EVFZ4
Chick embryo thymus homogenized directly in 2X Laemmli buffer, boiled 5 minutes.
The predicted MW of chicken histone H2AX is 15kDa the band migrates nearby at 14kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jenny Shoots
Verified customer
投稿 Jan 13 2016