Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
Asynchronous Hela were either methanol or paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody strongly recognizes a nuclear antigen that colocalizes with chromatin (DAPI staining). Peptide competition assays should be performed to confirm specificity of the antibody. The accompanying image is a field image of methanol fixed cells, but this antibody also works very well with paraformaldehyde fixed samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Oct 13 2008