Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (10T1/2)
Specification
10T1/2
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100 in PBS
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
Asynchronous 10T1/2 cells were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Specificity of the antibody should be further tested through peptide competition assays. The attached image is of a prometaphase cell.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Sep 12 2008