Application
Western blot
Sample
Human Cell lysate - whole cell (MRC 5VA - Lung Fibroblasts)
Loading amount
15 µg
Specification
MRC 5VA - Lung Fibroblasts
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Dako Polyclonal Rabbit anti-Mouse HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 30 kDa Non-specific: 70, 15 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 10 2009