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Products:Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Overview
Product name
Histone Acetyltransferase Activity Assay Kit (Colorimetric)
Cross reactivity
Reacts with
all Mammals
Product overview
The Histone Acetyltransferase (HAT) Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Shipped at 4°C. Store at -20ºC.
| Components | Identifier | 100 tests |
|---|---|---|
| 2X HAT Assay Buffer | Amber | 1 x 7.5ml |
| HAT Reconstitution Buffer | Clear | 1 x 1.8ml |
| HAT Substrate I | Blue | 1 vial |
| HAT Substrate II | Purple | 1 vial |
| NADH Generating Enzyme | Green | 1 x 800µl |
| Nuclear Extract (4 mg/ml ) | Red | 1 x 50µl |
Research areas
Epigenetics and Nuclear Signaling >> Assays & Kits >> Histone acetylation and deacetylation
Kits/ Lysates/ Other >> Kits >> Epigenetic kits >> Histone acetylation and deacetylation
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Amino Acid Assays
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
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Functional Studies - Histone Acetyltransferase Activity Assay Kit (Colorimetric) (ab65352)
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Relevance
Histone acetyltransferases (HAT) are enzymes that acetylate conserved lysine amino acids on histone proteins. They have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.
Cellular localization
Cytoplasmic and Nuclear. Note=Nuclear in S-phase cells and cytoplasmic.
Alternative names
- HAT
- HAT1
- Histone acetyltransferase 1
see all
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent dilution.
Histone Acetyltransferase Activity Assay Kit (Colorimetric) images:
Functional Studies - Histone Acetyltransferase Activity Assay Kit (Colorimetric) (ab65352)
Analysis of Histone Acetyltransferase Activity in HeLa Nuclear Extract using ab65352. HeLa nuclear extract in various amounts was incubated with HAT substrate. Activity was analyzed in a micro plate reader at 440 nm according to the kit instructions.
References for Histone Acetyltransferase Activity Assay Kit (Colorimetric) (ab65352)
This product has been referenced in:
- Masola Vet al. Heparanase and Syndecan-1 Interplay Orchestrates Fibroblast Growth Factor-2-induced Epithelial-Mesenchymal Transition in Renal Tubular Cells. J Biol Chem 287:1478-88 (2012).Read more (PubMed: 22102278) »
- Soliman ML & Rosenberger TA Acetate supplementation increases brain histone acetylation and inhibits histone deacetylase activity and expression. Mol Cell Biochem 352:173-80 (2011).Read more (PubMed: 21359531) »
See all 3 publications for this product
Publishing research using ab65352? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"