Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse prostate (Pten knockout))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
Permeabilization
No
Specification
Mouse prostate (Pten knockout)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% serum/pbs
Dilution
1/25
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
A range of dilutions tested (1:25-1:500) on mouse prostate. Pten knockout prostate is shown to have high expression of HKII by Western blotting, WT mouse prostate was used as a negative control. This antibody failed to produce positive staining in the Pten KO mouse prostate.
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with mouse and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Lucy Penfold
Verified customer
投稿 Nov 09 2016