Anti-Hexokinase 1 抗体 - Mitochondrial Outer Membrane Marker (ab65069)
Key features and details
- Rabbit polyclonal to Hexokinase 1 - Mitochondrial Outer Membrane Marker
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker
Hexokinase 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hexokinase 1 - Mitochondrial Outer Membrane Marker -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide derived from an internal sequence of human Hexokinase 1. [Swiss-Prot P19367]
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65069の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). |
ターゲット情報
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組織特異性
Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific. -
パスウェイ
Carbohydrate metabolism; hexose metabolism. -
関連疾患
Hexokinase deficiency
Neuropathy, hereditary motor and sensory, Russe type -
配列類似性
Belongs to the hexokinase family.
Contains 2 hexokinase domains. -
ドメイン
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule. -
細胞内局在
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. - Information by UniProt
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参照データベース
- Entrez Gene: 3098 Human
- Omim: 142600 Human
- SwissProt: P19367 Human
- Unigene: 370365 Human
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別名
- BB404130 antibody
- Brain form hexokinase antibody
- dea antibody
see all
画像
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All lanes : Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from HeLa cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069) at 1/500 dilution +
Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Exposure time: 4 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab65069 は 5 報の論文で使用されています。
- Li J et al. Renal protective effects of empagliflozin via inhibition of EMT and aberrant glycolysis in proximal tubules. JCI Insight 5:N/A (2020). PubMed: 32134397
- Sheikh MH et al. Immuno-metabolic impact of the multiple sclerosis patients' sera on endothelial cells of the blood-brain barrier. J Neuroinflammation 17:153 (2020). PubMed: 32386505
- Yu L et al. Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism. J Exp Clin Cancer Res 36:110 (2017). PubMed: 28810896
- Kishore M et al. Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis. Immunity 47:875-889.e10 (2017). PubMed: 29166588
- Tegnebratt T et al. Evaluation of efficacy of a new MEK inhibitor, RO4987655, in human tumor xenografts by [(18)F] FDG-PET imaging combined with proteomic approaches. EJNMMI Res 4:34 (2014). IHC . PubMed: 26116108