Application
ChIP
Sample
Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
Specification
haematopoietic progenitor cell line HPC-7
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.4% formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.4% formaldehyde
Detection step
Real-time PCR
Positive control
region known to bind this transcription factor
Negative control
region known to be devoid of this transcription factor
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
Here we used 14ug of antibody per 20,000,000 million cells. However, we have since repeated this experiment using 5-7ug of antibody and obtained the same result.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 12 2010