Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E14.5 Kidney)
Permeabilization
Yes - Triton X 100
Specification
E14.5 Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used:
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Additional data
Additional Notes
This antibody gave a lot of non-specific background staining on the Kidney sections. Additionally, we did not detect Hes1 staining in the nucleus of the E14.5 mouse kidney sections
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 07 2017