Application
Western blot
Sample
Human Cell lysate - whole cell (hepatoma cell lline)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Treatment
transfection of HCV core plasmid
Specification
hepatoma cell lline
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-mouse IgG-peroxidase antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 21 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 May 16 2016