Application
Western blot
Sample
Human Cell lysate - whole cell (HeK cells)
Loading amount
50 µg
Specification
HeK cells
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C
Secondary antibody
Name
Non-Abcam antibody was used: Sheep anti mouse
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 33, 36, 39 kDa
Positive control
lane 2 and 3 ( Hek cells transfected with pCI M only )
Negative control
lane 1 ( untransfected Hek cells)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Alina Macovei
Verified customer
投稿 Apr 15 2011