Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10 % gel)
Sample
Rat Tissue lysate - whole (aorta)
Specification
aorta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Bands
Specific: 32 kDa Non-specific: 25 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
The HO-1 bands are clear. For better quality, one can use 8% or lower gel.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 22 2013