Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Guinea pig Tissue lysate - whole (liver and brain)
Specification
liver and brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
17 hour(s) and 0 minute(s) · Diluent: 5 % BSA in TBST
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: cell signaling anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: > 40 kDa kDa
Exposure
7 minute(s) and 21 second(s)
Additional data
Additional Notes
It remains to be determined if the higher molecular weigth is due to species specific differences.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 02 2013