Anti-ureB 抗体 (ab127916)
Key features and details
- Rabbit polyclonal to ureB
- Suitable for: WB, IHC-P
- Reacts with: Helicobacter pylori
- Isotype: IgG
製品の概要
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製品名
Anti-ureB antibody
ureB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ureB -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Helicobacter pylori -
免疫原
Recombinant fragment corresponding to ureB aa 341-398.
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ポジティブ・コントロール
- ureB whole cell lysate; Mice Gastric pylori.
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特記事項
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab127916の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50000. Predicted molecular weight: 62 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. citrate buffer-10mM
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特記事項 |
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WB
1/50000. Predicted molecular weight: 62 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. citrate buffer-10mM |
ターゲット情報
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機能
Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach. -
パスウェイ
Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. -
配列類似性
Belongs to the urease family.
Contains 1 urease domain. -
翻訳後修飾
Carbamylation allows a single lysine to coordinate two nickel ions. -
細胞内局在
Cytoplasm. Also associates with the outer membrane upon autolysis of neighboring bacteria. - Information by UniProt
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別名
- HP_0072 antibody
- hpuB antibody
- URE1_HELPY antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab127916 は 4 報の論文で使用されています。
- Liu M et al. Oral immunization of mice with a multivalent therapeutic subunit vaccine protects against Helicobacter pylori infection. Vaccine 38:3031-3041 (2020). PubMed: 32139315
- Valenzuela-Valderrama M et al. The Helicobacter pylori Urease Virulence Factor Is Required for the Induction of Hypoxia-Induced Factor-1a in Gastric Cells. Cancers (Basel) 11:N/A (2019). PubMed: 31185594
- Ohishi T et al. Monotherapy with a novel intervenolin derivative, AS-1934, is an effective treatment for Helicobacter pylori infection. Helicobacter 23:e12470 (2018). WB . PubMed: 29488678
- Li N et al. Helicobacter pylori CagA promotes epithelial mesenchymal transition in gastric carcinogenesis via triggering oncogenic YAP pathway. J Exp Clin Cancer Res 37:280 (2018). PubMed: 30466467