Anti-Haspin 抗体 (ab21686)
Key features and details
- Rabbit polyclonal to Haspin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Haspin antibody
Haspin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Haspin -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Haspin.
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ポジティブ・コントロール
- This antibody gave a positive signal in the following whole cell lysates: HeLa Expressing myc-Haspin (interphase); HeLa Expressing myc-Haspin (mitosis) Whole Cell Lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21686の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 0.2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).
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特記事項 |
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WB
Use a concentration of 0.2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa). |
ターゲット情報
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機能
Required for normal alignment of chromosomes at metaphase. Phosphorylates histone H3 'Thr-3' during mitosis. -
組織特異性
Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated on both serine and threonine residues (By similarity). Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 83903 Human
- Omim: 609240 Human
- SwissProt: Q8TF76 Human
- Unigene: 534059 Human
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別名
- germ cell associated 2 antibody
- Germ cell-specific gene 2 protein antibody
- GSG2 antibody
see all
画像
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All lanes : Anti-Haspin antibody (ab21686) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : HeLa Expressing myc-Haspin (interphase) Whole Cell Lysate - Positive Control at 5 µl
Lane 3 : HeLa Interphase Whole Cell Lysate - Negative Control at 10 µg
Lane 4 : HeLa Expressing myc-Haspin (mitosis) Whole Cell Lysate - Positive Control at 5 µl
Lane 5 : HeLa Mitosis Whole Cell Lysate - Negative Control at 10 µg
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab21686 は 6 報の論文で使用されています。
- Huang Y et al. GSG2 Promotes Development and Predicts Poor Prognosis of Ovarian Cancer. Cancer Manag Res 13:499-508 (2021). PubMed: 33500663
- Yu F et al. Knockdown of GSG2 inhibits prostate cancer progression in vitro and in vivo. Int J Oncol 57:139-150 (2020). PubMed: 32319597
- Chen Y et al. GSG2 (Haspin) promotes development and progression of bladder cancer through targeting KIF15 (Kinase-12). Aging (Albany NY) 12:8858-8879 (2020). PubMed: 32439830
- Varier RA et al. A phospho/methyl switch at histone H3 regulates TFIID association with mitotic chromosomes. EMBO J 29:3967-78 (2010). PubMed: 20953165
- Markaki Y et al. Phosphorylation of histone H3 at Thr3 is part of a combinatorial pattern that marks and configures mitotic chromatin. J Cell Sci 122:2809-19 (2009). WB ; Human, Mouse . PubMed: 19622635
- Dai J et al. Studies of haspin-depleted cells reveal that spindle-pole integrity in mitosis requires chromosome cohesion. J Cell Sci 122:4168-76 (2009). WB ; Human . PubMed: 19910498