Anti-Hamartin 抗体 (ab25882)
Key features and details
- Rabbit polyclonal to Hamartin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Hamartin antibody
Hamartin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hamartin -
由来種
Rabbit -
特異性
This antibody will recognise both isoforms of TSC1. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide - a 15 amino acid peptide from the middle region of human TSC1 (Human). Peptide available as ab95277.
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ポジティブ・コントロール
- EL4 cell lysate can be used as positive control
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25882の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 2 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 2 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa). |
ターゲット情報
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機能
In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. -
組織特異性
Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells. -
関連疾患
Defects in TSC1 are the cause of tuberous sclerosis type 1 (TSC1) [MIM:191100]. It is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. TS1C is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
Defects in TSC1 may be a cause of focal cortical dysplasia of Taylor balloon cell type (FCDBC) [MIM:607341]. FCDBC is a subtype of cortical displasias linked to chronic intractable epilepsy. Cortical dysplasias display a broad spectrum of structural changes, which appear to result from changes in proliferation, migration, differentiation, and apoptosis of neuronal precursors and neurons during cortical development. -
ドメイン
The C-terminal putative coiled-coil domain is necessary for interaction with TSC2. -
翻訳後修飾
Phosphorylation at Ser-505 does not affect interaction with TSC2. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Membrane. At steady state found in association with membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 64930 Mouse
- SwissProt: Q9EP53 Mouse
- Unigene: 224354 Mouse
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別名
- Hamartin antibody
- kiaa0243 antibody
- LAM antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab25882 は 2 報の論文で使用されています。
- Hadley G et al. Investigation of the novel mTOR inhibitor AZD2014 in neuronal ischemia. Neurosci Lett 706:223-230 (2019). PubMed: 31100427
- Rosengren T et al. TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms. Cell Mol Life Sci 75:2663-2680 (2018). PubMed: 29396625