Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis)
Permeabilization
No
Specification
Testis
Blocking step
SuperBlock™ (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Bouin Soluion
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: SuperBlock™ (PBS) Blocking Buffer
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Thermo Fisher Scientific
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor 488
Additional data
Abcam response
Thank you for your valuable feedback about the use of ab65156 in IHC-Fr, an application we have not tested before.
We welcome any further feedback and AbReviews from other researchers who have used this antibody in IHC-Fr which will help us assess how well the antibody works in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kuo-Ting Chang
Verified customer
投稿 Nov 28 2017