Anti-HS6ST2 抗体 (ab122220)
Key features and details
- Rabbit polyclonal to HS6ST2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HS6ST2 antibody -
製品の詳細
Rabbit polyclonal to HS6ST2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: RKRQEQRKFL KGRLLQTHFQ SQGQGQSQNP NQNQSQNPNP NANQNLTQNL MQNLTQSLSQ KENRESPKQN SGKEQNDNTS NGTND, corresponding to amino acids 512-596 of Human HS6ST2.
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ポジティブ・コントロール
- Human cerebral cortex tissue; RT-4 and U-251 MG lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122220の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/250 - 1/500.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. |
ターゲット情報
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機能
6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. -
配列類似性
Belongs to the sulfotransferase 6 family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 90161 Human
- Omim: 300545 Human
- SwissProt: Q96MM7 Human
- Unigene: 385956 Human
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別名
- H6ST2_HUMAN antibody
- Heparan-sulfate 6-O-sulfotransferase 2 antibody
- HS6ST-2 antibody
see all
画像
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All lanes : Anti-HS6ST2 antibody (ab122220) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique. -
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122220 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122220, at 1/125 dilution, staining HS6ST2 in paraffin-embedded Human Cerebral cortex tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab122220 は 3 報の論文で使用されています。
- Zhang Y et al. Role of lncRNA FAM83H antisense RNA1 (FAM83H-AS1) in the progression of non-small cell lung cancer by regulating the miR-545-3p/heparan sulfate 6-O-sulfotransferase (HS6ST2) axis. Bioengineered 13:6476-6489 (2022). PubMed: 35260044
- Guo Y et al. Downregulation of HS6ST2 by miR-23b-3p enhances matrix degradation through p38 MAPK pathway in osteoarthritis. Cell Death Dis 9:699 (2018). PubMed: 29899528
- Jin Y et al. Overexpression of HS6ST2 is associated with poor prognosis in patients with gastric cancer. Oncol Lett 14:6191-6197 (2017). PubMed: 29113266