Application
Western blot
Sample
Human Cell lysate - whole cell (Colorectal Cancer Cells)
Gel Running Conditions
Reduced Denaturing (12% Gel)
Loading amount
200 µg
Specification
Colorectal Cancer Cells
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 27°C · Diluent: 2.5% Non-Fat Dry Milk
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: SCBT Goat anti Rabbit Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
GE ECL +
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 20 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 04 2010