PE Anti-HP1 alpha 抗体 [EPR5777] - Heterochromatin marker (ab210585)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
製品の概要
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製品名
PE Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker
HP1 alpha 一次抗体 製品一覧 -
製品の詳細
PE Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker -
由来種
Rabbit -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5777 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
- Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)
- HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721)
- Alexa Fluor® 647 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab198391)
- Alexa Fluor® 568 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab202533)
- Alexa Fluor® 594 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab203431)
- Alexa Fluor® 555 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab203432)
- Anti-HP1 alpha antibody [EPR5777] - BSA and Azide free (ab226049)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab210585の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/500.
The cellular localisation of this product has been verified in ICC/IF |
特記事項 |
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Flow Cyt (Intra)
1/500. The cellular localisation of this product has been verified in ICC/IF |
ターゲット情報
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機能
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. -
配列類似性
Contains 2 chromo domains. -
翻訳後修飾
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated. -
細胞内局在
Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. - Information by UniProt
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参照データベース
- Entrez Gene: 23468 Human
- Entrez Gene: 12419 Mouse
- Entrez Gene: 300266 Rat
- Omim: 604478 Human
- SwissProt: P45973 Human
- SwissProt: Q61686 Mouse
- Unigene: 349283 Human
- Unigene: 262059 Mouse
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別名
- Antigen p25 antibody
- CBX5 antibody
- CBX5_HUMAN antibody
see all
画像
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Overlay histogram showing HeLa cells stained with ab210585 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210585, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab210585 は論文での使用が確認できていません。