Application
ELISA
Sample
Dog Serum (NA)
Specification
NA
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Sandwich (Capture)
Other product details
Dilution
1/400
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
The antibody works extremely well in ELISA with reccombinant protein standard, but has low affinity for the canine protein.
Abcam response
Thank you for your collaboration in testing canine samples.
We have not tested canine and do not know canine sequence homology with Human.
We are happy to hear that there is some detection.
We have not tested canine and do not know canine sequence homology with Human.
We are happy to hear that there is some detection.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 10 2011