Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: LI-COR IRDye 800CW (827-08365)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Fluorescence
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 30 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. SAIFUDEEN ISMAEL
Verified customer
投稿 Aug 03 2017