Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6.0
Permeabilization
No
Specification
Liver
Blocking step
Leica Novolink kit (protein and peroxidase blocks) as blocking agent for 10 minute(s) · Concentration: 0µg/mL
Fixative
Formaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Leica Bond diluent
Dilution
1/500
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
1/1000 insufficient for liver, but also tested in healthy kidney and worked fine.
1/500 overnight worked well for our purposes in liver.
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投稿 Aug 17 2016