Application
ChIP
Sample
Rabbit Cell lysate - nuclear (endometrium)
Specification
endometrium
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Duration of cross-linking step: 0 second(s)
Detection step
Semiquantitative PCR
Positive control
PCR amplification of known postive sites.
Negative control
No antibody for the ChIP, which became a negative control in the PCR reaction, plus a water blank for the PCR reaction.
Other product details
Concentration
0.5 µg/µg chromatin
Incubation time
0 minute(s)
Additional data
Additional Notes
In vivo binding of SMARCA3 (RUSH) to DNA and Egr-1/c-Rel in the rabbit RUSH promoter was confirmed by PCR amplification of products from ChIP assays. Lane 1 shows the FX174/HaeIII size markers. Lane 2 shows the single 166-bp amplicons of the SMARCA3 (RUSH) site resulting from ChIP with antibodies to SMARCA3. Lanes 3 and 4 contain negative ChIP (IgG) and PCR (water) controls, respectively. Lane 5 shows the single 153-bp amplicons of the Sp1(A) site resulting from ChIP with antibodies to SMARCA3. The SMARCA3 (RUSH) site was identified through antibody-SMARCA3-DNA binding. The Sp1(A) site is an Egr-1/Sp1/3/MAZ/MZF1/c-Rel composite binding site that was identified through antibody-SMARCA3-Egr-1/c-Rel-DNA binding.
Cross-linking agent: disuccinimydyl glutarate and formaldehyde (0.75%)
Cross-linking agent: disuccinimydyl glutarate and formaldehyde (0.75%)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
PROF. Beverly Chilton
Verified customer
投稿 Aug 16 2006