Anti-HLA G 抗体 [5A6G7] (ab76869)
Key features and details
- Mouse monoclonal [5A6G7] to HLA G
- Suitable for: WB
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-HLA G antibody [5A6G7]
HLA G 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5A6G7] to HLA G -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
免疫原
Synthetic peptide corresponding to HLA G (C terminal) conjugated to ovalbumin. Amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 protein coupled to ovalbumin
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ポジティブ・コントロール
- WB: M8 cell line transfected with HLA-G5 and M8 cell line transfected with HLA-G6.
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特記事項
ab76869 allows discrimination between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified from TCS. Purity >95% by SDS-PAGE. -
一次抗体 備考
ab76869 allows discrimination between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs. -
ポリ/モノ
モノクローナル -
クローン名
5A6G7 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76869の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. |
ターゲット情報
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機能
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
組織特異性
Expressed in trophoblasts. -
配列類似性
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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別名
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
画像
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All lanes : Anti-HLA G antibody [5A6G7] (ab76869)
Lane 1 : M8 cell line transfected with empty vector
Lane 2 : M8 cell line transfected with HLA G1
Lane 3 : M8 cell line transfected with HLA G5
Lane 4 : M8 cell line transfected with HLA G6
Predicted band size: 38 kDa
Observed band size: 30,38 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab76869 は 9 報の論文で使用されています。
- Leisegang MS et al. The histone demethylase PHF8 facilitates alternative splicing of the histocompatibility antigen HLA-G. FEBS Lett 593:487-498 (2019). PubMed: 30758047
- Wan R et al. Human Leukocyte Antigen-G Inhibits the Anti-Tumor Effect of Natural Killer Cells via Immunoglobulin-Like Transcript 2 in Gastric Cancer. Cell Physiol Biochem 44:1828-1841 (2017). PubMed: 29224003
- Du MR et al. Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells. J Immunol 192:1502-11 (2014). Flow Cyt ; Human . PubMed: 24453244
- Guo Y et al. Soluble human leukocyte antigen-g5 activates extracellular signal-regulated protein kinase signaling and stimulates trophoblast invasion. PLoS One 8:e76023 (2013). WB ; Human . PubMed: 24098421
- Tuncel T et al. Immunoregulatory function of HLA-G in gastric cancer. Asian Pac J Cancer Prev 14:7681-4 (2013). PubMed: 24460353
- Gonzalez A et al. Identification of circulating nonclassic human leukocyte antigen G (HLA-G)-like molecules in exudates. Clin Chem 57:1013-22 (2011). PubMed: 21527645
- Rebmann V et al. Quantification and identification of soluble HLA-G isoforms. Tissue Antigens 69 Suppl 1:143-9 (2007). PubMed: 17445190
- Rebmann V et al. Report of the Wet Workshop for Quantification of Soluble HLA-G in Essen, 2004. Hum Immunol 66:853-63 (2005). PubMed: 16216668
- Le Rond S et al. Alloreactive CD4+ and CD8+ T cells express the immunotolerant HLA-G molecule in mixed lymphocyte reactions: in vivo implications in transplanted patients. Eur J Immunol 34:649-60 (2004). PubMed: 14991594