Application
Western blot
Sample
Human Cell lysate - whole cell (NPC)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
NPC
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP-linked Antibody
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 60 kDa Non-specific: 55, 45, 40, 35 kDa
Additional data
Additional Notes
The band is not expected as stated in datasheet, with the observed bands at around 60 kDa and too many non specific bands,
Abcam response
Thank you for your invaluable feedback. After reviewing the protocol details, we have offered an alternative antibody as requested, and we hope this replacement will give satisfactory results in your experiments.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 09 2017