Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Human Cell (Human endothelial cell line)
Specification
Human endothelial cell line
Permeabilization
Yes - 0.1% triton-x 100
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in PBS with Triton X-100 (0.1%)
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: bangalore genei
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 24 2013