Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (eye)
Specification
eye
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic
Blocking step
Other as blocking agent for 20 minute(s) · Concentration: 5%
Other product details
Dilution
1/50
Incubation time
5 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Dako Chemmate anti-mouse/anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Some positivity detected in the positive control, only in the most concentrated sample.
Abcam response
Thank you for your honest feedback. Since you are seeing some positive staining in your positive control, I believe you can acheive good results with some optimization of your protocol. You may wish to try a different antigen retrieval method, such as heat-mediated antigen retrieval with Tris-EDTA pH 9.0 buffer.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Luminita Paraoan
Verified customer
投稿 Nov 06 2006