Anti-HLA Class 1 ABC 抗体 [EMR8-5] (ab70328)
Key features and details
- Mouse monoclonal [EMR8-5] to HLA Class 1 ABC
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-HLA Class 1 ABC antibody [EMR8-5]
HLA Class 1 ABC 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [EMR8-5] to HLA Class 1 ABC -
由来種
Mouse -
特異性
ab70328 reacts with the heavy chains of human HLA class 1 A, -B, and -C. -
アプリケーション
適用あり: Flow Cyt, IHC-P, WBmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to HLA Class 1 ABC. Recombinant HLA-A*2402 extracellular domain
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ポジティブ・コントロール
- IHC-P: Human tonsil tissue sections
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EMR8-5 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70328の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | (1) |
Use 1-2µg for 106 cells.
(methanol or paraformaldehyde fixed cells) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P | (9) |
Use a concentration of 0.05 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (2) |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt
Use 1-2µg for 106 cells. (methanol or paraformaldehyde fixed cells) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 0.05 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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関連性
HLA-ABC antigen is present on most nucleated cells of humans and Old World monkeys. HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
細胞内局在
Cell Membrane; Single-pass type I membrane protein -
参照データベース
- Entrez Gene: 3105 Human
- Entrez Gene: 3106 Human
- Entrez Gene: 3107 Human
- SwissProt: P01889 Human
- SwissProt: P30443 Human
- SwissProt: P30499 Human
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別名
- Antigen Presenting Molecule antibody
- Cw*1701 antibody
- D6S204 antibody
see all
画像
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All lanes : Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)
Lane 1 : A*2402
Lane 2 : A*0101
Lane 3 : A*1101
Lane 4 : A*0201
Lane 5 : A*0207
Lane 6 : A2402
Lane 7 : B*0702
Lane 8 : B*0801
Lane 9 : B*1501
Lane 10 : B*3501
Lane 11 : B*4001
Lane 12 : B*4002
Lane 13 : B*4006
Lane 14 : B*4403
Lane 15 : Cw*0102
Lane 16 : Cw*0801
Lane 17 : Cw*1202
Lane 18 : Cw*1502Western blot analysis of ab70328 reactivity using recominant HLA heavy chains.
Lanes 1-6: HLA-A
Lanes 7-14: HLA-B
Lanes 15-18: HLA-C
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All lanes : Anti-HLA Class 1 ABC antibody [EMR8-5] (ab70328)
Lane 1 : Human oral cancer cell line OSC20
Lane 2 : HLA-A2402 gene transfected OSC20
Lane 3 : Human Leukemia cell line K562
Lane 4 : Recombinant HLA-A2402 heavy chain -
Overlay histogram showing Jurkat cells stained with ab70328 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70328, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
IHC image of HLA Class 1 ABC staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab70328, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (141)
ab70328 は 141 報の論文で使用されています。
- Godfrey J et al. Pembrolizumab for the treatment of disease relapse after allogeneic hematopoietic stem cell transplantation. Blood Adv 7:963-970 (2023). PubMed: 35973200
- Merckx C et al. Exploring the Therapeutic Potential of Ectoine in Duchenne Muscular Dystrophy: Comparison with Taurine, a Supplement with Known Beneficial Effects in the mdx Mouse. Int J Mol Sci 23:N/A (2022). PubMed: 36076964
- van der Woude LL et al. Tumor microenvironment shows an immunological abscopal effect in patients with NSCLC treated with pembrolizumab-radiotherapy combination. J Immunother Cancer 10:N/A (2022). PubMed: 36252995
- Mu D et al. Downregulation of PD-L1 and HLA-I in non-small cell lung cancer with ALK fusion. Thorac Cancer 13:1153-1163 (2022). PubMed: 35253386
- Xiong X et al. Emerging enterococcus pore-forming toxins with MHC/HLA-I as receptors. Cell 185:1157-1171.e22 (2022). PubMed: 35259335