Anti-HIV1 Vif 抗体 [319] (ab66643)
Key features and details
- Mouse monoclonal [319] to HIV1 Vif
- Suitable for: ICC/IF, WB
- Reacts with: Human immunodeficiency virus
- Isotype: IgG1
製品の概要
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製品名
Anti-HIV1 Vif antibody [319] -
製品の詳細
Mouse monoclonal [319] to HIV1 Vif -
由来種
Mouse -
特異性
ab66643 is specific for bacterial HIV1 Vif protein. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human immunodeficiency virus -
免疫原
Recombinant full length protein corresponding to HIV1 Vif. Bacterially expressed, hexahistidine amino-terminal tagged HIV1 Vif protein (clade B, HXB-3 isolate).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
319 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab66643の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/1000. Predicted molecular weight: 23 kDa.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 23 kDa. |
ターゲット情報
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関連性
HIV1 Vif (viral infectivity factor), supports productive viral replication in non-permissive cells, such as peripheral blood lymphocytes. It functions by inactivating a cellular anti-viral factor named APOBEC3G. Vif binds viral RNA and affects the stability of viral nucleoprotein core. May play a role in viral morphology. Interacts with host ABCE1, which seems to be involved in lentiviruses capsid formation and displays RNase L inhibitor activity. This interaction may play a role in protecting viral RNA from damage during viral assembly. -
参照データベース
- Entrez Gene: 155459 Human immunodeficiency virus
- SwissProt: P0C1K5 Human immunodeficiency virus
- SwissProt: Q77YG0 Human immunodeficiency virus
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別名
- SOR protein antibody
- Virion infectivity factor antibody
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プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab66643 は 9 報の論文で使用されています。
- Cai J et al. Infection with a newly designed dual fluorescent reporter HIV-1 effectively identifies latently infected CD4+ T cells. Elife 10:N/A (2021). PubMed: 33835029
- Koma T et al. The Expression Level of HIV-1 Vif Is Optimized by Nucleotide Changes in the Genomic SA1D2prox Region during the Viral Adaptation Process. Viruses 13:N/A (2021). PubMed: 34696508
- Gargan S et al. HIV-1 Promotes the Degradation of Components of the Type 1 IFN JAK/STAT Pathway and Blocks Anti-viral ISG Induction. EBioMedicine 30:203-216 (2018). WB . PubMed: 29580840
- Chen H et al. Anti-HIV Activities and Mechanism of 12-O-Tricosanoylphorbol-20-acetate, a Novel Phorbol Ester from Ostodes katharinae. Molecules 22:N/A (2017). PubMed: 28885587
- Desimmie BA et al. Identification of a tripartite interaction between the N-terminus of HIV-1 Vif and CBFß that is critical for Vif function. Retrovirology 14:19 (2017). PubMed: 28302150
- Brillen AL et al. Analysis of Competing HIV-1 Splice Donor Sites Uncovers a Tight Cluster of Splicing Regulatory Elements within Exon 2/2b. J Virol 91:N/A (2017). PubMed: 28446664
- Nomaguchi M et al. Production of HIV-1vifmRNA Is Modulated by Natural Nucleotide Variations and SLSA1 RNA Structure in SA1D2prox Genomic Region. Front Microbiol 8:2542 (2017). PubMed: 29326677
- Nakashima M et al. Structural Insights into HIV-1 Vif-APOBEC3F Interaction. J Virol 90:1034-47 (2015). PubMed: 26537685
- Widera M et al. A functional conserved intronic G run in HIV-1 intron 3 is critical to counteract APOBEC3G-mediated host restriction. Retrovirology 11:72 (2014). WB . PubMed: 25169827