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Synthetic peptide derived from within residues 37 - 86 of human HIPK2.
Our Abpromise guarantee covers the use of ab28507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 0.06 µg/ml. Predicted molecular weight: 110 kDa.Can be blocked with Human HIPK2 peptide (ab111675). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration. PubMed: 23565059|
Samples were incubated in primary antibody for 14 hours at 4°C.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"