Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
500 µg
Specification
293T
Immuno-precipitation step
Protein G
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk, 0.1% Tween-20, PBS
Western Blot antibody
Name
Non-Abcam antibody was used: Goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
This antibody works extremely well in IP experiments. 3 closely migrating bands of the predicted MW (approximately 90 kDa) were observed upon IP/IB. No such species were observed in the Control IgG IP. These multiple species are most likely modified forms of HIF-1Beta
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投稿 Oct 30 2007