Application
Western blot
Sample
Mouse Tissue lysate - whole (forebrain)
Loading amount
15 µg
Specification
forebrain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Concentration
0.25 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk TBS tween 0.1%
Secondary antibody
Name
Non-Abcam antibody was used: sheep anti mouse hrp amsersham
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL plus
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: ~90 kDa Non-specific: 60-70ish, >200 kDa
Additional data
Additional Notes
The approx 90 kDa band is clearly the strongest
Abcam response
Based on the other Abreviews, I suggest loading more cell lysate (20-50ug) and using more ab2771 (1:2000, 0.5ug/ml) to improve your results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Jun 22 2007