Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Endometrium)
Permeabilization
No
Specification
Endometrium
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
17 hour(s) and 0 minute(s) · Temperature: +4°C
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+R) Secondary Antibody, A
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
The sections were incubated with 10% normal goat serum for 60 min and then incubated with primary antibodies Anti-HIF-2-alpha (ab109616) at 5´g/ml, overnight at +4¯C.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Dominika Bartkowska
Verified customer
投稿 Apr 05 2017