Anti-HDAC7 抗体 [HDAC7-97] (ab50212)
Key features and details
- Mouse monoclonal [HDAC7-97] to HDAC7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-HDAC7 antibody [HDAC7-97]
HDAC7 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HDAC7-97] to HDAC7 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide:
YRTLEPLDTEGAARSVLSS
conjugated to KLH, corresponding to amino acids 137-155 of Mouse HDAC7 -
ポジティブ・コントロール
- WB: human embryonal kidney 293T cells expressing recombinant human HDAC7.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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特記事項(精製)
Ab50212 is a purified IgG. -
ポリ/モノ
モノクローナル -
クローン名
HDAC7-97 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50212の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 103 kDa.
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特記事項 |
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WB
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 103 kDa. |
ターゲット情報
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機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. -
配列類似性
Belongs to the histone deacetylase family. HD type 2 subfamily. -
ドメイン
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
翻訳後修飾
May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. -
細胞内局在
Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation. - Information by UniProt
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参照データベース
- Entrez Gene: 51564 Human
- Entrez Gene: 84582 Rat
- Omim: 606542 Human
- SwissProt: Q8WUI4 Human
- SwissProt: Q99P96 Rat
- Unigene: 200063 Human
- Unigene: 203327 Rat
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別名
- DKFZP586J0917 antibody
- FLJ99588 antibody
- HD 7a antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab50212 は 7 報の論文で使用されています。
- Mei J et al. OIP5-AS1 modulates epigenetic regulator HDAC7 to enhance non-small cell lung cancer metastasis via miR-140-5p. Oncol Lett 20:7 (2020). PubMed: 32774481
- Liang H et al. Orphan nuclear receptor NR4A1 suppresses hyperhomocysteinemia-induced hepatic steatosis in vitro and in vivo. FEBS Lett 593:1061-1071 (2019). PubMed: 30973961
- Malhotra R et al. HDAC9 is implicated in atherosclerotic aortic calcification and affects vascular smooth muscle cell phenotype. Nat Genet 51:1580-1587 (2019). PubMed: 31659325
- Shen W et al. Epigenetic modification of the leptin promoter in diet-induced obese mice and the effects of N-3 polyunsaturated Fatty acids. Sci Rep 4:5282 (2014). ChIP ; Mouse . PubMed: 24923522
- Muralidhar SA et al. Histone deacetylase 9 activates gamma-globin gene expression in primary erythroid cells. J Biol Chem 286:2343-53 (2011). WB ; Human . PubMed: 21078662
- Golding MC et al. Multiple epigenetic modifiers induce aggressive viral extinction in extraembryonic endoderm stem cells. Cell Stem Cell 6:457-67 (2010). ChIP . PubMed: 20452320
- Margariti A et al. Histone deacetylase 7 controls endothelial cell growth through modulation of beta-catenin. Circ Res 106:1202-11 (2010). PubMed: 20224040