Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain)
Loading amount
20 µg
Specification
Mouse brain
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Secondary antibody
Name
Non-Abcam antibody was used: Jackson ImmunResearch, #711-035-152
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 120 kDa Non-specific: 140, 95, 60 kDa
Additional data
Additional Notes
In our experiments/samples there were a number of different bands in the western blot, with the dominant band likely being non-specific. Care would have to be taken to ensure that the correct band is used for analysis. However, this may be an issue with the type of tissue we are using.
Abcam response
Thanks for your feedback on Anti-HDAC5 antibody ( ab55403 ) in mouse brain WB.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 07 2013