Application
Western blot
Sample
Human Cell lysate - whole cell (Peridontal ligament fibroblast)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
20 µg
Specification
Peridontal ligament fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/2000
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: ~50-60 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 15 2015