Application
Western blot
Sample
Mouse Tissue lysate - whole (lymphocyte)
Loading amount
500000 cells
Specification
lymphocyte
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/3000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk PBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Santa Cruz
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 55 kDa
Additional data
Abcam response
More concentrated antibody could be used in addition to lowering milk percentage in the antibody dilution buffer to get a stronger signal.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 19 2011