Application
ChIP
Sample
Human Cell lysate - whole cell (lymphoblastoid cell line)
Specification
lymphoblastoid cell line
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Real-time PCR
Positive control
p21 promoter
Negative control
beta actin coding region
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: ChIP dilution buffer
Additional data
Additional Notes
ChIP dilution buffer: 1% Triton X-100, 150mM NaCl, 2mM EDTA pH8, 20mM Tris pH8, 0.1% SDS, protease inhibitors.
The graphs shows the enrichment of the p21 promoter and beta actin coding region over GAPDH coding region, as measured by real time PCR
The graphs shows the enrichment of the p21 promoter and beta actin coding region over GAPDH coding region, as measured by real time PCR
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 24 2010