Application
Western blot
Sample
Mouse Tissue lysate - other (Sciatic nerve and spinal cord)
Loading amount
10 µg
Specification
Sciatic nerve and spinal cord
Gel Running Conditions
Reduced Denaturing (8-12%)
Blocking step
blocking buffer (infrared imaging system) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/300
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer plus 1:1000 tween 20
Secondary antibody
Name
Non-Abcam antibody was used: IR dye 680 goat-anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680
Dilution
1/1700
Detection
Detection method
LICOR
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 130, 100, 45 kDa
Positive control
tissue from mouse sciatic nerve and spinal cord
Additional data
Additional Notes
Lane 1 - mouse sciatic nerve
Lane 2 - mouse spinal cord
The same result was obtained with 10ug and 20ug protein and using a 1:500 and 1:300 dilution of primary antibody
Lane 2 - mouse spinal cord
The same result was obtained with 10ug and 20ug protein and using a 1:500 and 1:300 dilution of primary antibody
Abcam response
Our Scientific Support team have investigated this negative Abreview with the customer. We suggest that the customer has regrettably received a bad vial on this occasion, and they have been provided with a credit note.
We monitor complaints closely on a weekly basis, and we can confirm we have no further indication at present (26/3/12) that there are any general quality issues with this antibody or batch. This case will be kept on our quality monitoring records for future reference.
We monitor complaints closely on a weekly basis, and we can confirm we have no further indication at present (26/3/12) that there are any general quality issues with this antibody or batch. This case will be kept on our quality monitoring records for future reference.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Gillian Hunter
Verified customer
投稿 Mar 26 2012