Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - nuclear (B cells)
Specification
B cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Bands
Specific: 55 kDa
Exposure
30 second(s)
Additional data
Additional Notes
The last 4 lanes (next to the Molecular Weight Marker ladder) are loaded with cytoplasmic fractions, and the first 4 lanes are loaded with nuclear fractions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 16 2015