Application
ChIP
Sample
Mouse Tissue lysate - other (colonic crypts)
Specification
colonic crypts
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldhyde
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldhyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
3 µg/µg chromatin
Additional data
Additional Notes
treatment of isolated colonic crypts with Wnt3A for indicated times
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 06 2015