HDAC1 Sumoylation Assay Kit (ab115132)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell culture extracts, Tissue Extracts
製品の概要
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製品名
HDAC1 Sumoylation Assay Kit -
検出方法
Colorimetric -
サンプルの種類
Cell culture extracts, Tissue Extracts -
アッセイタイプ
Quantitative -
全工程の試験時間
3h 00m -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Mammals -
製品の概要
Sumoylation is a post-transcriptional modification involved in various cellular processes such as nuclear-cytoplasmic transport, transcription regulation and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2/-3 show a high degree of similarity to each other and are distinct from SUMO-1. Sumoylation is directed by an enzymatic cascade analogous to that involved in ubiquitination. Sumoylation of target proteins in vivo has been shown to cause a number of different outcomes, including altered localization and binding partners.
HDAC1 is a class I histone deacetylase that plays a critical role in transcriptional repression of gene expression and has been shown to be an integral component of multiprotein co-repressor complexes. It has been observed that an important mechanism of HDAC1 activity regulation is sumoylation, which acts by potentiating HDAC1 activity.
Abcam's HDAC1 Sumoylation Assay Kit (ab115132) allows the user to measure in vivo HDAC1 sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo HDAC1 sumoylation from multiple mammalian cells/tissues extracts.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 48 tests 96 tests 10X Wash Buffer 1 x 25ml 1 x 50ml 8-Well Assay Strips (with Frame) 6 units 12 units 8-Well Control Strips 2 units 3 units Color Development Solution 1 x 6ml 1 x 12ml Signal Enhancer 1 x 120µl 1 x 240µl Signal Report Solution 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml Sumo Antibody, 1µg/µL 1 x 5µl 1 x 10µl Sumo Assay Buffer 1 x 2ml 1 x 4ml Sumo Protein, 1 µg/µL 1 x 6µl 1 x 12µl -
研究分野
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別名
- GON 10
- HD1
- HDAC 1
see all -
参照データベース
- Entrez Gene: 3065 Human
- Entrez Gene: 433759 Mouse
- Entrez Gene: 297893 Rat
- Omim: 601241 Human
- SwissProt: Q13547 Human
- SwissProt: O09106 Mouse
- SwissProt: Q4QQW4 Rat
関連製品
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab115132 は論文での使用が確認できていません。