Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
40 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocker 1:1 in PBS + 0.1%Tween
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 680 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000
Detection
Detection method
LI-COR® Odyssey® detection system
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 88 kDa Non-specific: 65 kDa
Positive control
HA-tagged protein overexpression
Negative control
HeLa WCE
Additional data
Additional Notes
Picture legend.
1 - HeLa whole cell extract
2 - Overexpression of HA-tagged protein (4 mcg) in HeLa cells. Protein size is about 88 kDa.
1 - HeLa whole cell extract
2 - Overexpression of HA-tagged protein (4 mcg) in HeLa cells. Protein size is about 88 kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Svetlana Khoronenkova
Verified customer
投稿 Aug 17 2010