Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Transfected ES cells)
Specification
Transfected ES cells
Treatment
1uM all-trans retinoic acid for 24hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP : sc-2030
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 57+50 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
Untransfected cells
Additional data
Additional Notes
An HA tagged RARy (retinoic acid receptor gamma) protein was introduced in RARy knockout ES cells (RARy KO), and detected with the anti-HA antibody.
Conditions tested was presence (+) or absence (-) of 1uM all-trans retinoic acid (RA) for 24hrs.
PubMed ID
20857416: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 27 2015