Application
Flow Cytometry
Fixation
BD Fix/Perm
Permeabilization
Yes - BD Perm/Wash
Sample
Human Cell (293T)
Specification
293T
Gating Strategy
Gated on FSC-SSC to exclude debris/dead cells
Preparation
Cell harvesting/tissue preparation method: WT and cells expressing an HA-tagged protein (intracellular)
Sample buffer: BD fix/perm and perm/wash
Sample buffer: BD fix/perm and perm/wash
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: BD perm/wash
Dilution
1/200
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
BD cytofix/cytoperm used as per manufacturer protocol.
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投稿 Mar 11 2015