Anti-Grp75/MOT 抗体 (ab227215)
Key features and details
- Rabbit polyclonal to Grp75/MOT
- Suitable for: IP, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Grp75/MOT antibody
Grp75/MOT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Grp75/MOT -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Xenopus laevis -
免疫原
Recombinant fragment within Human Grp75/MOT (internal sequence). The exact sequence is proprietary.
Database link: P38646 -
ポジティブ・コントロール
- WB: HEK-293T, A431, HeLa, HepG2 and SCC-15 whole cell extract. IHC-P: Mouse duodenum tissue; rat duodenum and brain tissues. IP: HepG2 whole cell extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227215の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
1/100 - 1/500.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
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ICC/IF |
1/500.
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特記事項 |
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IP
1/100 - 1/500. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa. |
ICC/IF
1/500. |
ターゲット情報
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機能
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. -
配列類似性
Belongs to the heat shock protein 70 family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 416183 Chicken
- Entrez Gene: 517535 Cow
- Entrez Gene: 3313 Human
- Entrez Gene: 15526 Mouse
- Entrez Gene: 291671 Rat
- Entrez Gene: 379314 Xenopus laevis
- Omim: 600548 Human
- SwissProt: Q3ZCH0 Cow
see all -
別名
- 75 kDa glucose regulated protein antibody
- 75 kDa glucose-regulated protein antibody
- CSA antibody
see all
画像
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All lanes : Anti-Grp75/MOT antibody (ab227215) at 1/2500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded rat duodenum tissue stained for Grp75/MOT using ab227215 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence of HeLa cells staining Grp75/MOT with ab227215 (1/500). Hoechst 33342 staining. (blue).
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Anti-Grp75/MOT antibody (ab227215) at 1/500 dilution + SCC-15 (Human squamous cell carcinoma, tongue) whole cell extract at 6 µg
Predicted band size: 74 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded mouse duodenum tissue stained for Grp75/MOT using ab227215 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded rat brain tissue stained for Grp75/MOT using ab227215 at 1/500 dilution in immunohistochemical analysis.
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Grp75/MOT was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with 5 µg of ab227215. Western blot was performed from the immunoprecipitate using ab227215. Anti-Rabbit IgG was used as secondary antibody.
Lane 1: Control IgG instead of ab227215 in HepG2 whole cell extract.
Lane 2: ab227215 IP in HepG2 whole cell extract.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab227215 は論文での使用が確認できていません。